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Thermodynamics of Cyclodextrin Inclusion Complexes


Physical Chemistry is ubiquitous. The principles of spectroscopy can, for example, find applications in many disciplines. Fluorescence spectroscopy permits determination of the binding constant of a chromophore to a host molecule. Furthermore, by determining the binding constant as a function of temperature we can obtain the H and S of the complexation reaction. We have several objectives in this project. First, we are to determine the spectrum of the guest molecule and the host cyclodextrin molecule. Next we examine how the fluorescence spectrum changes when the host and guest are mixed. At the same time we will be studying about the chemistry of cyclodextrin and why it is important in biophysical chemistry.

Once we have the required spectra, we will obtain the equilibrium constant as a function of temperature and compute the thermodynamic properties of the complex between cyclodextrin and a chromophore.

At the end of the project we will produce a complete report in journal format.

Goals and Objectives


Background Chemistry of Cyclodextrins

Cyclodextrin and Its Complexes

Experimental Protocols

Fluorescence Spectroscopy Overview

Mathematical Interlude

Data Analysis




© Copyright 1999-2003, Theresa Julia Zielinski*, Alex Grushow**, Erica Harvey†, George M. Shalhoub‡, & the PCOL Consortium
Supported by the National Science Foundation 1999-2003

*Department of Chemistry, Medical Technology and Physics
Monmouth University
West Long Branch, NJ 07764-1898

**Department of Chemistry, Biochemistry & Physics
Rider University
2083 Lawrenceville Rd.
Lawrenceville, NJ 08648

†Erica Harvey
Department of Chemistry
Fairmont State College
Fairmont, WV 26554

‡Department of Chemistry and Biochemistry
LaSalle University
1900 West Olney Avenue
Philadelphia, PA 19141-1199

© All rights reserved.
You are welcome to use this document in your own classes but commercial use is not allowed without the permission of the author.

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The PCOL community acknowledges that partial support for this work was provided by the National Science Foundation's Division of Undergraduate Education through grant DUE #9950809. Additional support was provided by the Camille and Henry Dreyfus Foundation. PCOL faculty also acknowledge the National Science Teachers Association which awarded the PCOL Faculty Consortium the 1998 Gustav Ohaus Award for Innovation in College Science Teaching.

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The PCOL community acknowledges support from the Foundation for Independent Higher Education through a
2000-2001 AT&T Learning Network Teaching and Technology Grant

edited by

Theresa Julia Zielinski, Monmouth University

Mathcad documents contributed by Erica Harvey, Fairmont State College, Farimont, WV
George Shalhoub, LaSalle Universtity, Philadelphia, PA


Last updated for use at USC Upstate January 2015